Purification and specificity of bacteriolytic enzyme I from Hartmannella glebae

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منابع مشابه

Purification and specificity of bacteriolytic enzyme I from Hartmannella glebae.

A soil amoeba (Hartmannella glebae), when grown in conjunction with Enterobacter aerogenes and Alcaligenes faecalis, produced two enzymes. Enzyme I was purified by gel filtration on Sephadex G-100 and chromatography on diethylaminoethyl-cellulose. It is a basic protein. The analysis of the enzymic digest of the cell walls of Micrococcus lysodeikticus after reduction and acid hydrolysis showed t...

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Growth and bacteriolytic activity of a soil amoeba, Hartmannella glebae.

A soil amoeba, Hartmannella glebae, could grow on a variety of gram-positive and gram-negative bacteria, although the rate of growth was faster in the presence of gram-negative bacteria. The amoeba, however, could not use yeasts, molds, or a green alga as a nutritional source. The extract prepared from amoebae grown in the presence of Aerobacter aerogenes and Alcaligenes faecalis could lyse int...

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CARBOXYPEPTIDASE B I. PURIFICATION OF THE ZYMOGEN AND SPECIFICITY OF THE ENZYME* BY J. E. FOLKt

An enzyme capable of rapidly releasing carboxyl terminal lysine and arginine has been reported as an act,ive component of autolyzed bovine pancreas (2). In the same report it was recognized that this enzyme, carboxypeptidase B or basic carboxypeptidase, exists in fresh pancreas as an inactive zymogen which is activated by t,reatment of extra&s with trypsin. Although carboxypeptidase A’ is routi...

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Succinic Thiokinase. I. Purification of the Enzyme from Pig Heart.

Succinic thiokinase was found by Kaufman et al. (2, 3) and by Hift et al. (4) to be responsible for the substrate level phosphorylation reaction accompanying the oxidation of ar-ketoglutarate. The enzyme has been variously termed phosphorylation (“P”) enzyme (3, 4), GDP phosphorylation enzyme (5), succinic thiokinase (6-S), succinyl-CoA synthetase (9, lo), and succinate:CoA ligase (GDP) (11). T...

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synthesis of sulfides from alcohols and thiols in solvent-freeconditions and deoxygenation of sulfoxides

کاتالیست یک سنتز جدید برای تیواترها توصیف شده است. واکنش الکل ها با آریل، هتروآریل و آلکیل تیو ل ها درحضور 1،3،5- تری آزو- 2،4،6- تری فسفرین-2،2،4،4،6،6 هگزاکلراید ((tapc به عنوان یک کاتالیست موُثر، بازده های خوب تا عالی از تیواترها را حاصل می کند. علاوه براین، واکنش تحت شرایط بدون فلز و بدون حلال پیش می رود، بنابراین یک مکمل جالب برای روش های شناخته شده سنتز تیواترها ارائه می دهد. یک مکانیسم ا...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1977

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.33.1.1-5.1977