Purification and specificity of bacteriolytic enzyme I from Hartmannella glebae
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چکیده
منابع مشابه
Purification and specificity of bacteriolytic enzyme I from Hartmannella glebae.
A soil amoeba (Hartmannella glebae), when grown in conjunction with Enterobacter aerogenes and Alcaligenes faecalis, produced two enzymes. Enzyme I was purified by gel filtration on Sephadex G-100 and chromatography on diethylaminoethyl-cellulose. It is a basic protein. The analysis of the enzymic digest of the cell walls of Micrococcus lysodeikticus after reduction and acid hydrolysis showed t...
متن کاملGrowth and bacteriolytic activity of a soil amoeba, Hartmannella glebae.
A soil amoeba, Hartmannella glebae, could grow on a variety of gram-positive and gram-negative bacteria, although the rate of growth was faster in the presence of gram-negative bacteria. The amoeba, however, could not use yeasts, molds, or a green alga as a nutritional source. The extract prepared from amoebae grown in the presence of Aerobacter aerogenes and Alcaligenes faecalis could lyse int...
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An enzyme capable of rapidly releasing carboxyl terminal lysine and arginine has been reported as an act,ive component of autolyzed bovine pancreas (2). In the same report it was recognized that this enzyme, carboxypeptidase B or basic carboxypeptidase, exists in fresh pancreas as an inactive zymogen which is activated by t,reatment of extra&s with trypsin. Although carboxypeptidase A’ is routi...
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Succinic thiokinase was found by Kaufman et al. (2, 3) and by Hift et al. (4) to be responsible for the substrate level phosphorylation reaction accompanying the oxidation of ar-ketoglutarate. The enzyme has been variously termed phosphorylation (“P”) enzyme (3, 4), GDP phosphorylation enzyme (5), succinic thiokinase (6-S), succinyl-CoA synthetase (9, lo), and succinate:CoA ligase (GDP) (11). T...
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کاتالیست یک سنتز جدید برای تیواترها توصیف شده است. واکنش الکل ها با آریل، هتروآریل و آلکیل تیو ل ها درحضور 1،3،5- تری آزو- 2،4،6- تری فسفرین-2،2،4،4،6،6 هگزاکلراید ((tapc به عنوان یک کاتالیست موُثر، بازده های خوب تا عالی از تیواترها را حاصل می کند. علاوه براین، واکنش تحت شرایط بدون فلز و بدون حلال پیش می رود، بنابراین یک مکمل جالب برای روش های شناخته شده سنتز تیواترها ارائه می دهد. یک مکانیسم ا...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1977
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.33.1.1-5.1977